Multi-enzyme complexes on DNA scaffolds capable of substrate channeling with an artificial swinging arm
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Multi-enzyme complexes on DNA scaffolds capable of substrate channelling with an artificial swinging arm.
Swinging arms are a key functional component of multistep catalytic transformations in many naturally occurring multi-enzyme complexes. This arm is typically a prosthetic chemical group that is covalently attached to the enzyme complex via a flexible linker, allowing the direct transfer of substrate molecules between multiple active sites within the complex. Mimicking this method of substrate c...
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Substrate channeling is a process of transferring the product of one enzyme to an adjacent cascade enzyme or cell without complete mixing with the bulk phase. Such phenomena can occur in vivo, in vitro, or ex vivo. Enzyme-enzyme or enzyme-cell complexes may be static or transient. In addition to enhanced reaction rates through substrate channeling in complexes, numerous potential benefits of su...
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BACKGROUND The microbial synthesis of fuels, commodity chemicals, and bioactive compounds necessitates the assemblage of multiple enzyme activities to carry out sequential chemical reactions, often via substrate channeling by means of multi-domain or multi-enzyme complexes. Engineering the controlled incorporation of enzymes in recombinant protein complexes is therefore of interest. The cellulo...
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The structural properties of big multi enzyme complexes such as the pyruvate dehydrogenase complex result in unique and fascinating properties like self-assembly, high structural stability, coupled metabolic channeling within multi-step reactions and regeneration of co-factors. Since such properties are highly valuable for the engineering of artificial enzyme cascades, it is worthwhile to uncov...
متن کاملSwinging arms in multifunctional enzymes and the specificity of post-translational modification.
Introduction Covalently attached prosthetic groups serve as swinging arms in several different and wellcharacterized multifunctional enzymes. Notable among these are the lipoyl-lysine residues in 2-0x0 acid dehydrogenase multienzyme complexes [l-31 and the glycine cleavage system [4-61, the biotinyl-lysine residues in various ATP-dependent carboxylases [7,8] and the phosphopantetheinyl-serine r...
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تاریخ انتشار 2014